2. Adult mesenchymal stem cells biology and their clinical applications.
Our team has focused on adult stem cells (namely tissue specific mesenchymal stem cells, MSCs) identification and applications. We have developed novel cell culture methods for MSCs such as using rotary bioreactor; we reported the effects of cell differentiation on their immunogenicity properties; we were among the first groups reporting the identification and characterization of tendon derived stem cells (TDSCs); the use of TDSCs cell sheet for tendon repair; and the use of stem cell secretion factors for tissue engineering; we have also reported a simple method of cell de-differentiation to enhance their osteogenic potentials through epigenetic regulations and modifications of gene expressions.
PPT: Mesenchymal stem cells biology and their clinical applications.
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Representative Publications:
1. Yin Zi, Guo J, Wu TY, Chen X, Xu LL, Lin SE, Sun YX, Chan KM, Ouyang HW, Li G. Stepwise differentiation of mesenchymal stem cells augments tendon-like tissue formation and defect repair in vivo. Stem Cells Translational Medicine, 2016, in press.
2. Deng YJ, Li P, Li G, Qin L, Mak KK. Yap1 regulates multiple steps of chondrocyte differentiation during skeletal development and bone repair. Cell Reports, 2016 March 8; 14(9): 2224-2237.
3. Rui YF, Xu LL, Chen R, Zhang T, Lin S, Hou YH, Liu Y, Meng FB, Liu ZQ, Ni M, Tsang KZ, Yang FY, Wang C, Chan HC, Jiang XH, Li G. Epigenetic memory gained by priming with osteogenic induction medium improves osteogenesis and other properties of mesenchymal stem cells. Scientific Reports, 2015; 5:11056(1-15).
4. Xu LL, Liu Y, Hou YH, Wang KX, Wong YM, Lin SE, Li G. U0126 promotes osteogenesis of rat bone marrow-derived mesenchymal stem cells by activating BMP/Smad signaling pathway. Cell & Tissue Research, 2015; 359(2): 537-545.
5. Zhang XB, Meng XM, Baylink D, Neises A, Kiroyan J, Su RJ, Kimberly P, Gridley D, Wang J, William KH, Lee W, Li G. Rapid and efficient reprogramming of human fetal and adult blood CD34+ cells into mesenchymal stem cells with a single factor. Cell Research, 2013; 23(5):658-72.
6. Fan QM, Yue B, Bian ZY, Xu WT, Tu B, Dai KR, Li G, Tang TT. The CREB–Smad6–Runx2 axis contributes to the impaired osteogenesis potential of bone marrow stromal cells in fibrous dysplasia of bone. Journal of Pathology, 2012; 228(1):45-55
7. Rui YF, Lui PP, Li G, Fu SC, Lee YW, Chan KM. Isolation and characterization of multipotent rat tendon-derived stem cells. Tissue Engineering Part A 2010; 16(5):1549-58.
8. Chen X, McClurg A, Zhou GQ, McCaiguea M, Armstrong MA, Li G. Chondrogenic differentiation alters the immunosuppressive property of bone marrow-derived mesenchymal stem cells, and the effect is partially due to the upregulated expression of B7 molecules. Stem Cells; 2007; 25:364-370.
9. Chen X, Xu H, Wan C, McCaigue M, Li G. Bioreactor expansion of human adult bone marrow-derived mesenchymal stem cells (MSCs). Stem Cells; 2006; 24: 2052-2059.
10. Wan C, He Q, McCaige M, Marsh D, Li G. Nonadherent cell population of human marrow culture is a complementary source of mesenchymal stem cells (MSCs). Journal of Orthopaedic Research; 2006; 24 (1): 21-28. |